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KMID : 0380020080230050425
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Korean Journal of Biotechnology and Bioengineering
2008 Volume.23 No. 5 p.425 ~ p.430
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The MALDI-TOF MS determination of yeast proteins producing H2S
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Abstract
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Hydrogen sulfide (H2S) is a by-product of metabolism of amino acids including sulfur and alcoholic fermentation, it is generally thought of in terms of a poisonous gas. Though H2S can have a negative impact on the perceived quality of fermented drinks due to an undesirable aroma, it plays prominent roles as a neuromodulator in the mammalian brain as well as a smooth muscle relaxant. Nowadays studies on the proteins which produce H2S are carried out in various fields such as structure, function, and metabolism. Here we propose to develop a simple and rapid H2S forming assay method, which will lead to speed up preparing the H2S forming proteins for identification by MALDI-TOF MS analysis. We detected three kinds of proteins which produce H2S in the crude extract of Saccharomyces cerevisiae. Those proteins were cystathionie β-synthase, O-acetylserine sulfhydrylase, and cystathionine γ-lyase.
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KEYWORD
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Hydrogen sulfide, cystathionine β-synthase, O-acetylserine sulfhydrylase, MALDI-TOF MS analysis
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